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Insulin-Like Growth Factor I, Recombinant, Human (IGF1, IGF I)
Insulin-Like Growth Factor I, Recombinant, Human (IGF1, IGF I)
Pricing
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| Recombinant Human IGF-1 is a single, non-glycosylated, polypeptide chain containing 70aa and having a molecular weight of 7,655Da. The sequence of the first five N-terminal amino acids was determined to be Gly-Pro-Glu-Thr-Leu. N-terminal methionine has been completely removed enzymatically. Contains less than 1% dimers and aggregates. | | | | | | Source: E. coli | | | Catalog # | I7661-11E | | Molecular Weight | ~7.65kD | | Endotoxin | 0.1 ng/ug | | Activity | | | The ED50 as determined by the dose-dependent proliferation of murine Balb/c 3T3 cells (measured by H-thymidine uptake) is <1.0ng/ml, corresponding to a specific activity of 1x10U/mg. For most in vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20ng/ml. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Stable for at least 6 months at -20°C. Reconstitute with sterile deionized water to a concentration 100ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~7.65kD | | Source | E. coli | | Purity | 98% (HPLC, FPLC and reducing and non-reducing SDS-PAGE). | | Form | Supplied as a lyophilized powder from 50mM acetic acid. No preservative added. Reconstitute with sterile deionized water to a concentration 100ug/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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