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Insulin-Like Growth Factor Binding Protein 3, Recombinant Human (IGFBP3, tcag7.703, BP-53; IBP3, IGF-binding protein 3, Growth hormone-dependent binding protein)
Insulin-Like Growth Factor Binding Protein 3, Recombinant Human (IGFBP3, tcag7.703, BP-53;
IBP3, IGF-binding protein 3, Growth hormone-dependent binding protein)
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| Insulin Like Growth Factor Binding Protein-3 is one of the six homologous proteins that specifically bind insulin-like growth factors and modulate their mitogenic and metabolic actions. IGFBP-3 is the major IGF binding protein present in serum of humans and animals. It is also present in the alpha granules of platelets. IGFBP-3 shows a similar affinity for IGF-1 and IGF-2. IGFBP-3 inhibits follicle stimulating hormone . Markedly decreased levels of IGFBP-3 are observed in patients with growth hormone deficiencies, while markedly elevated levels are observed in patients with high levels of growth hormone (acromegaly). In murine fibroblasts the synthesis of IGFBP-3 is stimulated by mitogenic growth factors such as bombesin, vasopressin, PDGF, and EGF. In human skin fibroblasts the synthesis of IGFBP-3 is stimulated by TGF-beta. | | | Catalog # | I7661-16J1 | | Recombinant Human IGFBP-3 is a single, non-glycosylated polypeptide chain containing 264 amino acids and having a molecular mass of 28.8kD. | | Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Ala-Ser-Ser-Gly. | | Biological Activity | IGFBP-3 is fully biologically active when compared to standards. The ED50, calculated by by its ability to inhibit IGF-II induced proliferation of MCF-7 is less then 0.2ug/ml in the presence of 15ng/ml of Human IGF-II. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile dH2O, 0.1% HSA or BSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 28.8kD | | Source | E. coli | | Purity | 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. | | Form | Supplied as a sterile filtered lyophilized powder in 20mM Tris HCl. Reconstitute in sterile ddH2O, 0.1%HSA or BSA to 0.1mg/ml, which can then be further diluted to other aqueous solutions. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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