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You are here:Home » Molecular Biology » Growth Factors-IGF » Insulin-Like Growth Factor Binding Protein 3, Recombinant Human (IGFBP3, tcag7.703, BP-53; IBP3, IGF-binding protein 3, Growth hormone-dependent binding protein)

Insulin-Like Growth Factor Binding Protein 3, Recombinant Human (IGFBP3, tcag7.703, BP-53;
IBP3, IGF-binding protein 3, Growth hormone-dependent binding protein)

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Specifications

Insulin Like Growth Factor Binding Protein-3 is one of the six homologous proteins that specifically bind insulin-like growth factors and modulate their mitogenic and metabolic actions. IGFBP-3 is the major IGF binding protein present in serum of humans and animals. It is also present in the alpha granules of platelets. IGFBP-3 shows a similar affinity for IGF-1 and IGF-2. IGFBP-3 inhibits follicle stimulating hormone . Markedly decreased levels of IGFBP-3 are observed in patients with growth hormone deficiencies, while markedly elevated levels are observed in patients with high levels of growth hormone (acromegaly). In murine fibroblasts the synthesis of IGFBP-3 is stimulated by mitogenic growth factors such as bombesin, vasopressin, PDGF, and EGF. In human skin fibroblasts the synthesis of IGFBP-3 is stimulated by TGF-beta.
Catalog #I7661-16J1
Recombinant Human IGFBP-3 is a single, non-glycosylated polypeptide chain containing 264 amino acids and having a molecular mass of 28.8kD.
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Gly-Ala-Ser-Ser-Gly.
Biological ActivityIGFBP-3 is fully biologically active when compared to standards. The ED50, calculated by by its ability to inhibit IGF-II induced proliferation of MCF-7 is less then 0.2ug/ml in the presence of 15ng/ml of Human IGF-II.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile dH2O, 0.1% HSA or BSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight28.8kD
SourceE. coli
Purity98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
FormSupplied as a sterile filtered lyophilized powder in 20mM Tris HCl. Reconstitute in sterile ddH2O, 0.1%HSA or BSA to 0.1mg/ml, which can then be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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