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You are here:Home » Molecular Biology » Growth Factors-IGF » IGFBP-7, Recombinant, Mouse, Western Blot Positive Control (Insulin-like Growth Factor-binding Protein 7, IBP-7, IGF-binding Protein 7, IBP7, GFBP-rP1, MAC25 Protein, PGI2-stimulating Factor, Prostacyclin-stimulating Factor, Tumor-derived Adhesion Factor,

IGFBP-7, Recombinant, Mouse, Western Blot Positive Control (Insulin-like Growth
Factor-binding Protein 7, IBP-7, IGF-binding Protein 7, IBP7, GFBP-rP1, MAC25 Protein,
PGI2-stimulating Factor, Prostacyclin-stimulating Factor, Tumor-derived Adhesion Factor,

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Specifications

Unlike insulin, both IGF-I and IGF-II circulate in plasma tightly bound to specific binding proteins. Two major forms of IGF-binding proteins have been identified in human plasma, a low molecular weight form and a high molecular weight form. The low molecular weight IGF-binding protein (IGFBP1) is synthesized in liver, secretory endometrium, and decidua. It binds both IGF I and IGF II with high affinity. Insulin-like growth factor binding protein-1 (IGFBP-1 ~30kD; also known as IBP1 or placental protein 12, or IGF-BP25; chromosome 7p14-p12)) is a member of the superfamily of insulin-like growth factor (IGF) binding proteins which include six high-affinity IGF binding proteins (IGFBP1-6) and at least four low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). The IGFBP members are cysteine- rich proteins with conserved cysteine residues, clustered in the amino-terminal and the carboxy-terminal regions of the molecule. Contained within IGFBP-1 and -2 is an integrin receptor recognition sequence (RGD) that is responsible for promoting cell migration by an IGF-independent action. IGFBPs hold a central position in IGF ligand-receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. IGFBPs will either inhibit or enhance the biological activities of IGF or act in an IGF-independent manner.
Catalog #I7661-17F1
IGF-BP7 is expressed in a wide range of normal human tissues and it generally shows reduced expression in cancer cell lines of prostate, breast, colon, and lung origin. It plays a role in skeletal myogenesis by binding to IGF in a manner that inhibits IGF induced differentiation of skeletal myoblasts, without affecting IGF induced proliferation. Additionally, IGF-BP7 suppresses growth and colony formation of prostate and breast cancer cell lines through an IGF independent mechanism, which causes a delay in the G1 phase of the cell cycle, and increased apoptosis. Recombinant human IGF-BP7 is a 26.4kD protein consisting of 257aa residues. IGFBP-RP1 is closely related to CTGF (IGFBP-8), NOV (IGFBP-9) and CYR61 (IGFBP-10). The four proteins constitute a subfamily of low-affinity IGFBPs.
SourceRecombinant mouse IGFBP-7 (28-281aa), expressed in Sf21 cells as human CD33-His tag at the N-terminus.
Molecular Weight~35kD
ApplicationsSuitable for use in Western Blot. Not suitable for use in ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~35kD
SourceSf21 cells
PurityHighly Purified (~95%)
ConcentrationNot determined.
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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