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You are here:Home » Molecular Biology » Growth Factors-IGF » Insulin-Like Growth Factor I Receptor, Recombinant, Human, GST-Tag (IGF1R)

Insulin-Like Growth Factor I Receptor, Recombinant, Human, GST-Tag (IGF1R)

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Specifications

Human IGF1R (GenBank Accession No. NP_000875). GST fusion protein containing the Mr 46 kD cytoplasmic domain of the b-subunit of the human Insulin-like Growth Factor-1 Receptor Kinase, residues 930-1337, expressed in a baculovirus expression system.
Catalog #I7661-20J
Specific Activity~100 nmol/min/mg at 25°C using poly(Glu:Tyr) 4:1 substrate. Reaction conditions are 50mM Tris, pH 7.5, 5mM MgCl2, 5mM MnCl2, 1mM DTT, 250uM ATP. The kinase concentration was 0.5-1uM.
ApplicationsEnzyme kinetics, autophosphorylation, and phosphorylation of target substrates.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Molecular Weight72.3kD
SourceBaculovirus infected Sf9 cells
Purity80% by SDS-PAGE
Concentration~1mg/ml
FormSupplied as a liquid in 50mM Tris, pH 7.5 and 1mM DTT.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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