| The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a b-a-a-b configuration. The b-subunit (95kD) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the a-subunit (135kD) is completely extracellular. |
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| Catalog # | I7661-26HX |
| Applications | Suitable for use in Affinity Purification, Conjugation, ELISA, Western Blot, Tyrosine Kinase Assay and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications not tested. |
| Recommended Dilutions | Affinity Purification: With peptide elution. |
| Tyrosine Kinase Assay: Antibody mediated capture on microtiter plates |
| Western Blot: 1-2ug/ml for 2hrs at RT |
| Immunohistochemistry (Formalin/paraffin): 1:50 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes. |
| Optimal dilution to be determined by researcher. |
| Positive Control | HEP-G-2, IM-9 lymphocyte cells, placenta or brain. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3F225 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins and no preservatives added. Also available with BSA and azide. See I7661-26H. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant fragment including the C-terminal 100 amino acids of human insulin receptor. Cellular Localization: Cell membrane. |
| Specificity | Recognizes human Insulin Receptor, beta (95kD). Species Crossreactivity: monkey, mouse and rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
