Insulin Receptor, beta, Recombinant, Human, GST-Tag CAS

Grade

Purified

Swiss Prot

P06213

Accession Number

NM_000208

Molecular Weight

71.7

EU Commodity Code

30021019

Shipping Temp

Dry Ice

Storage Temp

-70°C

Human INSR, Insulin receptor (beta) (GenBank Accession No. NM_000208). GST fusion protein containing the Mr 48kD cytoplasmic domain of the b-subunit of the human insulin receptor, residues 941-1343, expressed in a baculovirus expression system.

Specific Activity

~117 nmol/min/mg @ 25°C using poly(Glu:Tyr) 4:1 substrate. Reaction conditions are 50mM Tris, pH 7.5, 30mM MgCl2, 1 mM DTT, 1.0mM ATP and 1mg/ml substrate.

Applications

Suitable for use in Enzyme kinetics, autophosphorylation, and phosphorylation of target substrates. Other applications not tested.

Storage and Stability

Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Source

Baculovirus infected Sf9 cells

Purity

≥ 80% by SDS-PAGE

Concentration

~1mg/ml

Form

Supplied as a liquid in 50mM Tris, pH 7.5 and 1mM DTT.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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I7661-26K Insulin Receptor, beta, Recombinant, Human, GST-Tag (IRb) CAS:

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