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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified FC

The OX-62 antigen has recently been cloned and shown to be the rat alpha E2 integrin, which is expressed by dendritic epidermal T cells, intraepithelial lymphocytes in the small intestine and by cells of dendritic morphology present at sites where gamma delta T cells occur in lymphoid organs.
Catalog #	I7661-30E
Applications	Suitable for use in Flow Cytometry. Other applications have not been tested.
Recommended Dilution	Flow Cytometry: Neat. Use 10ul to label 10e6 cells in 100ul.
	Optimal dilution to be determined by the researcher.
Hybridoma	Spleen cells of BALB/c mice fused with mouse NSO myeloma cells.
Recommended Negative Control	I1904-79C: IgG1 Murine Negative Control (For rat tissue) (PE)
Storage and Stability	Store lyophilized powder at 4°C. Do Not Freeze. Reconstitute with 1 ml distilled water and store at 4°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H2471
Host	Mouse
Source	Rat
Concentration	Not determined
Form	Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with R. Phycoerythrin (RPE). Reconsitute with 1ml distilled water.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Density gradient enriched PVG rat thoracic duct dendritic cells.
Specificity	Recognizes rat OX62 antigen expressed by some rat dendritic cells and by gamma delta T cells.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.