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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin, alpha1, beta1 (CD49a, VLA alpha1, Laminin Receptor, Collagen Receptor)

Anti -Integrin, alpha1, beta1 (CD49a, VLA alpha1, Laminin Receptor, Collagen Receptor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC
CD49a is the integrin alpha1 chain. It is non-covalently associated with the 130kD integrin beta1 chain (CD29 molecule). CD49a amd CD29 form the VLA1 complex (1). It is known as laminin and collagen receptor. It is expressed by activated T lymphocytes, monocytes, melanoma cells, smooth muscle cells and broblasts. It is found on microvascular endothelial cells and neuroblastomas. VLA-1 is a receptor for collagen and laminin.
Catalog #I7661-31B
ApplicationsSuitable for use in Immunoprecipitation of VLA-1 integrin from detergent cell lysates, Flow Cytometry, Immunohistochemistry. Not recommended for Western Blot. Other applications not tested.
Recommended DilutionImmunoprecipitation: 2ug/10e6 cell equivalent (Detergent cell lysates).
Flow Cytometry: 10ug/ml.
Immunohistochemistry: 20ug/ml. Detects VLA-1 in acetone-fixed frozen tissue sections from gut, heart and lungs.
Optimal dilution determined by the researcher.
HybridomaFusion of spleen cells from Balb/c mice with myeloma P3X63ag9.653 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No2Q1015
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRat pheochromocytoma PC12 cells
SpecificityDirected against rat integrin a1b1 (VLA-1). Specificity was confirmed by ability to immunoprecipitate alpha1 (Mr~200kD) and beta1 (Mr~120kD) from 125I-surface labeled PC12 cells as well as reciprocal depletion of VLA-1 antigen from cell lysate with antiserum against the cytoplasmic domain of a1 integrin subunit. Does not inhibit VLA-1 mediated adhesion to matrix proteins.
Species CrossreactivityRat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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