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You are here:Home » Antibodies » Antibodies-Integrin » Anti -Integrin alpha1 I

Anti -Integrin alpha1 I


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH FC
Catalog #I7661-31C
ApplicationsSuitable for use in ELISA, Immunohistochemistry, Westen Blot, Immunoprecipitation, Flow Cytometry and Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: 2ug/ml, with paraformaldehyde fixation.
Western Blot: 2ug/ml
Immunoprecipitation: 1ug/ml
Flow Cytometry: 2ug/ml
ELISA: 0.5ug/ml
Inhibition: 1-5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No2Q1420
FormSupplied as a liquid in PBS, pH 7.1, 0.15M sodium chloride. No preservatives added.
PurityPurified by Protein A Sepharose chromatography.
ImmunogenIntegrin alpha 1 I
SpecificityRecognzes the I domain (Val151ľAla364) of human alpha1 integrin (CD49a). Precipitates proteins of Mr 180kD, pI 5.9-6.0 (alpha chain) and Mr 115kD, pI 4.5-5.0 (beta chain) from human lymphocyte lysates. Does not recognize rat recombinant I domain of integrin alpha1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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