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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alpha1 I

Anti -Integrin alpha1 I

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH FC
Catalog #I7661-31D
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry and Inhibition assays. Other applications not tested.
Recommended DilutionsELISA: 0.5ug/ml
Western Blot: 2ug/ml
Immunohistochemistry: 2ug/ml with paraformaldehyde fixation.
Immunoprecipitation: 1ug/ml
Flow Cytometry: 2ug/ml
Inhibition Assay: 1-5ug/ml. Using activated human lymphocyte binding to laminin, collagen IV and fibronectin.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q1420
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
PurityPurified by Protein A Sepharose chromatography.
ImmunogenHuman Integrin alpha1 I
SpecificityRecognizes the I domain (Val151ľAla364) of human a1 integrin (CD49a). Precipitates proteins of Mr 180kD, pI 5.9-6.0 (alpha chain) and Mr 115kD, pI 4.5-5.0 (beta chain) from human lymphocyte lysates. Does not recognize rat recombinant I domain of integrin a1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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