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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alpha2, beta1 (Collagen Receptor, VLA2) (Azide Free)

Anti -Integrin alpha2, beta1 (Collagen Receptor, VLA2) (Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
Does not detect alpha 2 integrin subunit by Western Blot presumably due to conformational properties of the epitope involved. The epitope is dependent on the presence of the I-domain of alpha 2 integrin subunit and does not bind VLA-2 variant which lacks the I-domain.
Catalog #I7661-32B
ApplicationsSuitable for use in Immunoprecipitation, Flow Cytometry, Immunohistochemistry, and Inhibition of VLA-2 mediated cell adhesion to collagen and laminin. Other applications not tested.
Recommended DilutionImmunoprecipitation: 2-5ug (lysate of 10e6 cell equivalent)
Flow Cytometry: 10ug/ml
Immunohistochemistry (Frozen tissue sections): 5ug/ml
Inhibition: 10ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaP3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No2Q1003
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman fibrosarcoma HT1080 cells.
SpecificityRecognizes the human collagen receptor VLA-2 (alpha 2 beta 1) integrin complex. Species crossreactivity: porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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