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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alpha3 (CD49c, Very Late Antigen3a chain, VLA3a)

Anti -Integrin alpha3 (CD49c, Very Late Antigen3a chain, VLA3a)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP IH IF RIA
Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 19 alpha and 8 beta subunits that form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. Both of the subunits contribute to the binding of the ligand. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit.
Catalog #I7661-33A
ApplicationsSuitable for use in ELISA, RIA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.
Recommended DilutionsELISA/RIA: 1:1000
Western Blot: 1:500 Detects Intergrin alpha 3 on 10ug of A431 lysates.
Immunoprecipitation: 5ul precipitates alpha3/beta1 from 5x10e6 cells.
Immunohistochemistry/Immunofluorescence: 1:500; acetone-fixed tissue sections
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-39 IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Positive ControlHuman fetal foreskin fibroblasts (HFFF) neuroblastomas
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to the C-terminal sequence, CRIQPSETERLTDDY, located in the cytoplasmic domain of the human alpha 3 integrin subunit (NM_002204.1).
SpecificityRecognizes human Integrin alpha3 at ~130kD. Specificity determined by immunoprecipitation from a detergent extract of 35S-methionine labeled neuroblastoma cells. No crossreactivity to alpha1, alpha2, alpha4, alpha5, alpha6 or alphaV integrins. Species Crossreactivity: mouse, rat, bovine, equine, sheep, porcine, canine, chicken, goat and hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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