| Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 19 alpha and 8 beta subunits that form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. Both of the subunits contribute to the binding of the ligand. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. |
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| Catalog # | I7661-33A |
| Applications | Suitable for use in ELISA, RIA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications have not been tested. |
| Recommended Dilutions | ELISA/RIA: 1:1000 |
| Western Blot: 1:500 Detects Intergrin alpha 3 on 10ug of A431 lysates. |
| Immunoprecipitation: 5ul precipitates alpha3/beta1 from 5x10e6 cells. |
| Immunohistochemistry/Immunofluorescence: 1:500; acetone-fixed tissue sections |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies I1904-39 | IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Positive Control | Human fetal foreskin fibroblasts (HFFF) neuroblastomas |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid, 0.05% sodium azide. |
| Purity | Serum |
| Immunogen | Synthetic peptide corresponding to the C-terminal sequence, CRIQPSETERLTDDY, located in the cytoplasmic domain of the human alpha 3 integrin subunit. |
| Specificity | Recognizes human Integrin alpha3 at ~130kD. Specificity determined by immunoprecipitation from a detergent extract of 35S-methionine labeled neuroblastoma cells. No crossreactivity to alpha1, alpha2, alpha4, alpha5, alpha6 or alphaV integrins. Species Crossreactivity: mouse, rat, bovine, equine, sheep, porcine, canine, chicken, goat and hamster. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
