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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alpha3, beta1 (CD49c, Very Late Antigen3a1b chain, VLA3a1b)

Anti -Integrin alpha3, beta1 (CD49c, Very Late Antigen3a1b chain, VLA3a1b)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IP IH IC FC RIA
Integrins are a family of dimeric proteins that mediate cell-cell and extracellular matrix adhesion. Each VLA subtype (VLA-1, -2, -3, -4 and -5) is composed of a distinct alpha subunit noncovalently associated with a common beta subunit, as an alpha-beta dimer. Integrin VLA-3 (very late antigen, alpha 3/beta 1) is a cell surface receptor that binds to fibronectin, lamin, collagen type I and VI. VLA-3 is typically restricted in normal tissue but is widely expressed in many cultured tumor cells and correlates with the degree of invasiveness of malignant melanoma cells.
Catalog #I7661-33C
ApplicationsSuitable for use in Immunohistochemistry, ELISA, RIA, Immunoprecipitation, Immunocytochemistry and FACS. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: Acetone fixed, paraffin embedded sections
Immunoprecipitation: Under non-reducing conditions, two chains with apparent molecular weight of 110 and 150kD. In the presence of a reducing agent (2% B-ME), a single molecular species of 130kD is detected. Under reducing conditions both alpha3 and beta1 subunits run with the same apparent molecular weight.
Optimal dilutions to be determined by the researcher.
Positive ControlsCell Line: KJ29 renal carcinoma adherent cells
Tissue: Kidney. Reacts with basal layer podocytes and basement membrane of distal tubules.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q993
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman renal carcinoma cell line (KJ29).
SpecificityRecognizes human Integrin alpha3, beta1. Recognizes an epitope related to the VLA-3 complex (a3/b1 integrin). Specificity was determined by immunoprecipitation from a detergent extract of 35S-methionine labeled KJ29 cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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