| Integrins are a family of dimeric proteins that mediate cell-cell and extracellular matrix adhesion. Each VLA subtype (VLA-1, -2, -3, -4 and -5) is composed of a distinct alpha subunit noncovalently associated with a common beta subunit, as an alpha-beta dimer. Integrin VLA-3 (very late antigen, alpha 3/beta 1) is a cell surface receptor that binds to fibronectin, lamin, collagen type I and VI. VLA-3 is typically restricted in normal tissue but is widely expressed in many cultured tumor cells and correlates with the degree of invasiveness of malignant melanoma cells. |
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| Catalog # | I7661-33C |
| Applications | Suitable for use in Immunohistochemistry, ELISA, RIA, Immunoprecipitation, Immunocytochemistry and FACS. Other applications have not been tested. |
| Recommended Dilution | Immunohistochemistry: Acetone fixed, paraffin embedded sections |
| Immunoprecipitation: Under non-reducing conditions, two chains with apparent molecular weight of 110 and 150kD. In the presence of a reducing agent (2% B-ME), a single molecular species of 130kD is detected. Under reducing conditions both alpha3 and beta1 subunits run with the same apparent molecular weight. |
| Optimal dilutions to be determined by the researcher. |
| Positive Controls | Cell Line: KJ29 renal carcinoma adherent cells |
| Tissue: Kidney. Reacts with basal layer podocytes and basement membrane of distal tubules. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 2Q993 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, 0.05% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Human renal carcinoma cell line (KJ29). |
| Specificity | Recognizes human Integrin alpha3, beta1. Recognizes an epitope related to the VLA-3 complex (a3/b1 integrin). Specificity was determined by immunoprecipitation from a detergent extract of 35S-methionine labeled KJ29 cells. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
