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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alpha5, beta1 (VLA5, Fibronectin Receptor, Very Late Antigen, CD49)

Anti -Integrin alpha5, beta1 (VLA5, Fibronectin Receptor, Very Late Antigen, CD49)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Ascites B IP FC
Specificity of the antibody was verified by its ability to immunoprecipitate a5b1 heterodimers from 125-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express a5b1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions.
Catalog #I7661-35P
ApplicationsSuitable for use in the Inhibition of cell adhesion to fibronectin, Immunoprecipitation, Flow Cytometry and Western Blot. Other applications not tested.
Recommended DilutionsInhibition of Cell Adhesion to Fibronectin: 10ul/ml
Note: where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (a4b1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete.
Immunoprecipitation from lysate of 10e6 cell equivalent: 2ul Flow Cytometry: 10ul/ml
Western Blot: Non-denaturing gel
Optimal dilution determined by the researcher.
HybridomaFusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG3
Clone No2Q962
HostRat
SourceMouse
ConcentrationNot Determined
FormSupplied as a liquid.
PurityAscites
ImmunogenMouse embryonal carcinoma P19 cells
SpecificityRecognizes mouse Integrin alpha5, beta1, a fibronectin receptor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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