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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alpha5, beta1 (VLA5, Fibronectin Receptor, Very Late Antigen, CD49)

Anti -Integrin alpha5, beta1 (VLA5, Fibronectin Receptor, Very Late Antigen, CD49)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Ascites B IP FC
The alpha 5 beta 1 integrin mediates cell adhesion and migration on fibronectin, a glycoprotein critical for normal vertebrate embryonic development. Indirect evidence reported to date suggests that this receptor also functions in the deposition of fibronectin matrices.
Catalog #I7661-35S
ApplicationsSuitable for use in Immunoprecipitation and Flow Cytometry. Other applications have not been tested.
Recommended DilutionFlow Cytometry: 1:100 or 10ul per 10e6 cells.
Does not detect VLA-5 (alpha 5 beta 1) in Western blot and tissue samples. Inhibition of VLA-5 mediated cell adhesion to fibronectin is generally achieved at 10ul per 100ul of cell suspension containing 10e6 cells.
Immunoprecipitation: 5ul of BMA5 in no more than 500ul cell lysate (<0.5% triton X-100 recommended).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No2Q965
HostRat
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid, no preservatives
PurityAscites
ImmunogenIntegrin alpha 5, beta 1.
SpecificityReacts with the fibronectin receptor alpha 5 beta 1 complex. Species Crossreactivity: Mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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