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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B IP IH IC

Integrins are a family of heterodimeric membrane glycoproteins consisting on non-covalently associated alpha and beta subunits. In general, integrins function as receptors for extracellular matrix proteins.
Catalog #	I7661-36C
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.
	Other applications have not been tested.
Recommended Dilutions
	Western Blot: Recognizes a 130kD band in chicken brain tissue and a 140kD band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation).
	Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor.
	Immunocytochemistry: Methanol-fixed cultured cells (15 minute fixation).
	Immunohistochemistry (Methanol-fixed cryosections): 35ug/ml An overnight methanol fix is suggested.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	2Q958
Host	Mouse
Source	Chicken
Concentration	~1mg/mkl
Form	Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Purity	Purified
Immunogen	Integrin alpha subunits purified from adult chicken brain tissue, denatured in 6M guanidine isothiocyanate, and depleted of beta1 subunit
Specificity	Recognizes chicken integrin alpha6, as demonstrated by experiments with cDNA-transfected NIH3T3 cells followed by immunoblots.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.