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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alpha6 (CD49f)

Anti -Integrin alpha6 (CD49f)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Supernatant B IH FC
Catalog #I7661-36C1
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. In Western Blot reactive with two major bands of 120 and 140kD under non-reducing conditions. Other applications not tested.
Recommended DilutionImmunohistochemistry (Frozen): 1:5-1:10 Dilute with PBS with 1% BSA and 0.1% sodium azide. Incubate for 30 min. at RT or overnight at 4°C.
Optimal dilutions to be determined by the researcher.
Positive ControlEpithelial tissues
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5F200
HostRat
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid.
PurityCulture supernatant.
ImmunogenMouse mammary tumor cells.
SpecificityRecognizes mouse Integrin alpha6. In cutaneous melanocytic lesions, the level of expression of three integrin subunits (a2, a6, and b3) has been found to be correlated with tumor progression. I7661-36C1 is directed against a6-integrins (Laminin receptor, VLA-6), shows a cytoplasmic staining pattern of uveal and cutaneous melanoma cells. Antibody blocks binding of integrin a6 to laminin P1 and E8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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