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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alpha6 (VLA6, alpha6, beta1, alpha6 beta4, Laminin Receptors)

Anti -Integrin alpha6 (VLA6, alpha6, beta1, alpha6 beta4, Laminin Receptors)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IP IH IC FC
Catalog #I7661-36D
ApplicationsSuitable for use in Immunoprecipitation, Flow Cytometry, Immunohistochemistry. Not suitable for use in Western Blot since the epitope most likely involves both alpha 6 and beta 1 integrin subunit. Does not block alpha 6 integrin mediated cell adhesion to laminin. Activation of integrin signaling properties by MA6 has not been characterized. Other applications not tested.
Recommended DilutionImmunoprecipitation: 2ug of MA6. (10e6 cell lysate).
Flow Cytometry: 10ug/ml
Immunohistochemistry: 20ug/ml (frozen tissue sections).
Immunocytochemistry Cell lines tested by using antibody to express alpha 6 integrins include: mouse bone marrow-derived mast cells, melanoma cell line B16F1, squamous carcinoma SCC-VII, fibroblast cell line NIH-3T3, splenic T lymphocytes, thymocytes and various T cell lines.
HybridomaFusing rat spleen cells withMouse SCC-VII squamous cell carcinoma.
Cells lines that express Integrin alpha6Mouse bone marrow-derived mast cells, melanoma cell line B16F1, ssquamous carcinoma SCC-VII, fibroblast cell line NIH-3T3, splenic T-lymphocytes, thymocytes and various T-cell lines.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2,k
Clone No2Q956
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH7.6, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenIntegrin VLA-6, alpha 6.
SpecificityReacts with mouse laminin receptor alpha 6 beta 1 and alpha 6 beta 4 complexes. Species crossreactivity: Mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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