Anti -Integrin alpha6 (VLA6, alpha6, beta1, alpha6 beta4, Laminin Receptors)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
IP IH IC FC |
|
| Catalog # | I7661-36D | | Applications | Suitable for use in Immunoprecipitation, Flow Cytometry, Immunohistochemistry. Not suitable for use in Western Blot since the epitope most likely involves both alpha 6 and beta 1 integrin subunit. Does not block alpha 6 integrin mediated cell adhesion to laminin. Activation of integrin signaling properties by MA6 has not been characterized. Other applications not tested. | | Recommended Dilution | Immunoprecipitation: 2ug of MA6. (10e6 cell lysate). | | Flow Cytometry: 10ug/ml | | Immunohistochemistry: 20ug/ml (frozen tissue sections). | | Immunocytochemistry | Cell lines tested by using antibody to express alpha 6 integrins include: mouse bone marrow-derived mast cells, melanoma cell line B16F1, squamous carcinoma SCC-VII, fibroblast cell line NIH-3T3, splenic T lymphocytes, thymocytes and various T cell lines. | | Hybridoma | Fusing rat spleen cells withMouse SCC-VII squamous cell carcinoma. | | Cells lines that express Integrin alpha6 | Mouse bone marrow-derived mast cells, melanoma cell line B16F1, ssquamous carcinoma SCC-VII, fibroblast cell line NIH-3T3, splenic T-lymphocytes, thymocytes and various T-cell lines. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2,k | | Clone No | 2Q956 | | Host | Rat | | Source | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH7.6, 0.1% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Integrin VLA-6, alpha 6. | | Specificity | Reacts with mouse laminin receptor alpha 6 beta 1 and alpha 6 beta 4 complexes. Species crossreactivity: Mouse | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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