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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alpha8

Anti -Integrin alpha8

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IC FC
Integrin alpha8 is a 170-200kD member of the integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with integrin beta-1. Alpha-8/beta-1 romotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse integrin alpha-8 is a 1025aa type I transmembrane glycoprotein. It contains a 969aa extracellular region (aa38-1006) and a 29aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin alpha8 shares 96% and 90% aa sequence identity with rat and human Integrin alpha-8 protein, respectively.
Catalog #I7661-36V
ApplicationsSuitable for use in Western Blot, Immunocytochemistry, Flow Cytometry and Direct ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1ug/ml
Immunocytochemistry: 5-15ug/ml used to detect Integrin alpha8 in immersion fixed 4T1 mouse mammary carcinoma cells.
Flow Cytometry: 2.5ug labels 10e6 4T1 mouse mammary carcinoma cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to Phe38-Phe1007 from mouse Integrin alpha8, expressed in CHO cells (NP_001001309).
SpecificityRecognizes mouse Integrin alpha8. Shows <1% crossreactivity with recombinant human and mouse Integrin alpha5 and recombinant human and mouse Integrin alphaV in direct ELISA and Western Blot. Species Sequence Homology: rat (96%), human (90%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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