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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IC FC

Integrin alpha8 is a 170-200kD member of the integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with integrin beta-1. Alpha-8/beta-1 romotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse integrin alpha-8 is a 1025aa type I transmembrane glycoprotein. It contains a 969aa extracellular region (aa38-1006) and a 29aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin alpha8 shares 96% and 90% aa sequence identity with rat and human Integrin alpha-8 protein, respectively.
Catalog #	I7661-36V
Applications	Suitable for use in Western Blot, Immunocytochemistry, Flow Cytometry and Direct ELISA. Other applications not tested.
Recommended Dilutions	Western Blot: 0.1ug/ml
	Immunocytochemistry: 5-15ug/ml used to detect Integrin alpha8 in immersion fixed 4T1 mouse mammary carcinoma cells.
	Flow Cytometry: 2.5ug labels 10e6 4T1 mouse mammary carcinoma cells.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Mouse
Concentration	~0.2mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Recombinant corresponding to Phe38-Phe1007 from mouse Integrin alpha8, expressed in CHO cells (NP_001001309).
Specificity	Recognizes mouse Integrin alpha8. Shows <1% crossreactivity with recombinant human and mouse Integrin alpha5 and recombinant human and mouse Integrin alphaV in direct ELISA and Western Blot. Species Sequence Homology: rat (96%), human (90%).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.