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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alpha8 (Biotin)

Anti -Integrin alpha8 (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IC FC
Integrin alpha 8 is a 170-200kD member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin b1. Alpha8beta1 promotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGFb1 and nephronectin. Mouse Integrin b8 is a 1025 aa type I transmembrane glycoprotein. It contains a 969aa extracellular region (aa38-1006) and a 29aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin a8 shares 96% and 90% aa sequence identity with rat and human Integrin a8 protein, respectively.
Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.
Catalog #I7661-36V1
Recommended DilutionsFlow cytometry: 50ug/ml; 10ul labels 1-2.5x10e5 cells in a total reaction volume 200ul. Tested on 4T1 mouse mammary carcinoma cells.
Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions. In this format, shows <5% crossreactivity with recombinant human Integrin alpha5 and recombinant mouse Integrin alpha5, and <1% crossreactivity with recombinant mouse Integrin alphaV.
Immunocytochemistry: 10ug/ml detects Integrin alpha8 in 4T1 mouse mammary carcinoma cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted I7661-36V1 for 3 hours at RT followed by a fluorochrome-conjugated streptavidin at RT for 1 hour. Between each step, cells were washed with PBS, 0.1% BSA.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 0.25% BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS, pH 7.3, 0.1% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa38-1007 from the extracellular domain of mouse Integrin alpha8, expressed in CHO cells (NP_001001309).
SpecificityRecognizes the extracellular domain of mouse Integrin alpha 8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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