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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin, alpha9 (Integrin alpha4-like)

Anti -Integrin, alpha9 (Integrin alpha4-like)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B FC
Integrin alpha9 is a 150kD type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta1 integrin. Alpha9beta1 binds to VEGF-C and D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse integrin alpha9 contains three beta-propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha9 ECD is absolutely conserved in mouse alpha9 ECD (Arg 566-Val 567). The ECD of mouse integrin alpha9 shares 95% and 89% aa sequence identity to rat and human integrin alpha9, respectively.
Catalog #I7661-36Y1
ApplicationsSuitable for use in Western Blot, Flow Cytometry and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1ug/ml. Tested on recombinant mouse Integrin alpha9.
Flow Cytometry: 2.5ug labels 10e6 cells. Tested on D3 mouse embryonic stem cells.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant protein corresponding to Try31-Val979 from mouse Integin alpha9 expressed in NSO cells (NP_598482).
SpecificityRecognizes mouse Integrin alpha9. Shows <5% crossreactivity with recombinant human Integrin alpha1, alpha8, alpha9, alphaL, recombinant mouse Integrin 3, 4, 5, E and M.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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