| Integrin alpha9 is a 150kD type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta1 integrin. Alpha9beta1 binds to VEGF-C and D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse integrin alpha9 contains three beta-propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha9 ECD is absolutely conserved in mouse alpha9 ECD (Arg 566-Val 567). The ECD of mouse integrin alpha9 shares 95% and 89% aa sequence identity to rat and human integrin alpha9, respectively. |
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| Catalog # | I7661-36Y1 |
| Applications | Suitable for use in Western Blot, Flow Cytometry and ELISA. Other applications not tested. |
| Recommended Dilutions | Western Blot: 0.1ug/ml. Tested on recombinant mouse Integrin alpha9. |
| Flow Cytometry: 2.5ug labels 10e6 cells. Tested on D3 mouse embryonic stem cells. |
| Optimal dilutions should be determined by each laboratory for each application. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Mouse |
| Concentration | ~0.2mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Recombinant corresponding to mouse Integin alpha9 expressed in NSO cells (Try31-Val979, Accession #NP_598482) |
| Specificity | Recognizes mouse Integrin alpha9. Shows <5% crossreactivity with recombinant human Integrin alpha1, alpha8, alpha9, alphaL, recombinant mouse Integrin 3, 4, 5, E and M. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
