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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin, alpha9 (Integrin, alpha4-like)

Anti -Integrin, alpha9 (Integrin, alpha4-like)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Integrin alpha 9 is a 150kD type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the b1 Integrin. a9b1 binds to VEGF-C and D, and osteopontin. The 951aa extracellular domain of mouse Integrin a9 contains three b-propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human a9 ECD is absolutely conserved in mouse a9 ECD (Arg 566-Val 567). The ECD of mouse Integrin a9 shares 95% and 89% aa sequence identity to rat and human Integrin a9, respectively.
Catalog #I7661-36Z
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.5-1ug/ml
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaMouse myeloma cells with B cells obtained from a mouse/rat immunized with purified, E. coli-derived, recombinant human Integrin a9 (aa89-169 and aa501-557).
Storage and StabilityLyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9L835
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified, E. coli-derived, recombinant human Integrin a9 (aa89-169 and aa501-557; Accession # Q13797).
SpecificityRecognizes human Integrin alpha 9. Species sequence homology: mouse (89%), rat (95%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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