| The involvement of integrins in vascular proliferation, adhesion and wound repair and have been well-documented. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen and von Willebrand factor (6). Due to its involvement in angiogenesis, the integrin aVb3 receptor is one of the most intensely studied of the integrin receptors. |
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| Catalog # | I7661-37E1 |
| Applications | Suitable for use in Flow Cytometry, Immunoprecipitation and Imunofluorescence. Not effective for Immunohistochemical staining of paraffin-embedded tissue sections. Other applications not tested. |
| Recommended Dilution | Immunofluorescence: Inhibits adhesion of cells to vitronectin coated surfaces at 10-25ug/ml. |
| Optimal dilutions to be determined by the researcher. |
| Absorption Maximum | 490nm, 545nm and 565nm |
| Emission Maximum | 580nm |
| Positive Control | Human myeloma cell lines M21 and MoalphaV |
| Negative Control | Mo cells (this cell line was derived from M21 but does not express integrin alphaV) |
| Storage and Stability | May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 2Q880 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.6, 0.05% sodium azide, 0.01% Thimerosal, 15mg/ml BSA. Conjugated to R-Phycoerythrin (PE). |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Purified adhesion receptor from the human melanoma cell line M21. |
| Specificity | Recognizes human vitronectin receptor aVb3 complex, an RGD-directed adhesion receptor. Species Crossreactivity: Avian, bovine, canine, chicken, monkey, porcine and rabbit. Not reactive with rat tissue. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
