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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alphaV, beta3 (PE)

Anti -Integrin alphaV, beta3 (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP FC IF
The involvement of integrins in vascular proliferation, adhesion and wound repair and have been well-documented. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen and von Willebrand factor (6). Due to its involvement in angiogenesis, the integrin aVb3 receptor is one of the most intensely studied of the integrin receptors.
Catalog #I7661-37E1
ApplicationsSuitable for use in Flow Cytometry, Immunoprecipitation and Imunofluorescence. Not effective for Immunohistochemical staining of paraffin-embedded tissue sections. Other applications not tested.
Recommended DilutionImmunofluorescence: Inhibits adhesion of cells to vitronectin coated surfaces at 10-25ug/ml.
Optimal dilutions to be determined by the researcher.
Absorption Maximum490nm, 545nm and 565nm
Emission Maximum580nm
Positive ControlHuman myeloma cell lines M21 and MoalphaV
Negative ControlMo cells (this cell line was derived from M21 but does not express integrin alphaV)
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q880
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.05% sodium azide, 0.01% Thimerosal, 15mg/ml BSA. Conjugated to R-Phycoerythrin (PE).
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified adhesion receptor from the human melanoma cell line M21.
SpecificityRecognizes human vitronectin receptor aVb3 complex, an RGD-directed adhesion receptor. Species Crossreactivity: Avian, bovine, canine, chicken, monkey, porcine and rabbit. Not reactive with rat tissue.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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