| The involvement of integrins in vascular proliferation, adhesion, and wound repair and have been well-documented. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (3). Due to its involvement in angiogenesis, the integrin aVb3 receptor is one of the most intensely studied of the integrin receptors. |
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| Catalog # | I7661-37E2 |
| Applications | Suitable for use in Immunoprecipitation, Immunofluorescence and Inhibition of adhesion. Not suitable for Immunohistochemical staining of paraffin-embedded tissue sections. Other applications have not been tested. |
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| Recommended Dilution: |
| Inhibition of adhesion of cells to vitronectin coated surfaces: 10-25ug/ml |
| Immunohistochemistry/Immunofluorescence: Frozen |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 2Q880 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS pH 7.6, 0.1% sodium azide, 15mg/ml BSA, before the addition of glycerol to 40%. Labeled with FITC. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Purified adhesion receptor from the human melanoma cell line M21. |
| Specificity | Recognizes the human vitronectin receptor aVb3 complex, an RGD-directed adhesion receptor. Species Crossreactivity: bovine, chick, monkey, porcine, canine, rabbit and avian. Not reactive with rat tissue. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
