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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E IP IC FC

Catalog #	I7661-37P
Applications	Immunoprecipitation
	Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4C in 20% normal human serum then washing in medium containing 10% FBS. 5X105 cells per tube were subsequently incubated with primary antibody for 30 min on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody.
	Immunocytochemistry: 15mg/ml dilution for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation for 30 minutes at 22C, followed by detection with a fluorescein-conjugated secondary antibody.
	ELISA
Storage and Handling	Maintain at 2–8°C in undiluted aliquots for up to 12 months
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	2Q873
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS. No preservative added.
Purity	Purified immunoglobulin
Immunogen	Generated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.
Specificity	Reactive with human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. Binds to the alphaVbeta5 complex. Has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding to alphaVbeta5. Does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.