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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alphaV, beta5

Anti -Integrin alphaV, beta5

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E IP IC FC
Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions.
Catalog #I7661-37P
ApplicationsSuitable for use in ELISA, Immunoprecipitation, Flow Cytometry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4ºC in 20% normal human serum then washing in medium containing 10% FBS. 5x10e5 cells per tube were subsequently incubated with primary antibody for 30 minutes on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody.
Immunocytochemistry: 15mg/ml for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation for 30 minutes at 22ºC, followed by detection with a fluorescein-conjugated secondary antibody.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2Q873
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.1. No preservative added.
PurityPurified
ImmunogenHuman integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.
SpecificityRecognizes human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. Binds to the alphaVbeta5 complex. Has a Kd of 2.8nM in Mn2+, 4.0nM in Ca2+and 2.9nM in buffer lacking cation. Thus, cations do not significantly affect the binding to alphaVbeta5. Does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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