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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alphaV, beta5

Anti -Integrin alphaV, beta5

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E IP IC FC
Catalog #I7661-37P
ApplicationsImmunoprecipitation
Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4C in 20% normal human serum then washing in medium containing 10% FBS. 5X105 cells per tube were subsequently incubated with primary antibody for 30 min on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody.
Immunocytochemistry: 15mg/ml dilution for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation for 30 minutes at 22C, followed by detection with a fluorescein-conjugated secondary antibody.
ELISA
Storage and Handling Maintain at 28°C in undiluted aliquots for up to 12 months
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2Q873
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS. No preservative added.
PurityPurified immunoglobulin
ImmunogenGenerated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.
SpecificityReactive with human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. Binds to the alphaVbeta5 complex. Has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding to alphaVbeta5. Does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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