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You are here:Home » Antibodies » Antibodies-Integrin » Anti -Integrin alphaV, beta6

Anti -Integrin alphaV, beta6


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP FC
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumors. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alphaV beta6 is upregulated in cancers and during tissue remodelling but is absent from resting adult tissues. Integrin alphaV beta6 promotes invasion and correlates with poor survival and therefore makes a promising therapeutic target.
Catalog #I7661-37W
ApplicationsSuitable for use in Flow Cytometry, Immunoprecipitation and Function Blockcing. Not suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry (mouse and human): Use buffers containing 1mM Mg, since the epitope recognized is divalent cation-dependent.
Immunoprecipitation: Effective. Protein G or rabbit anti-mouse IgG recommended for capture.
Function Blocking: Inhibition of alphaV beta6-mediated cell adhesion and cell migration are achieved at a concentration of 100ug/ml using beta6 transfected cells tested against TGFbeta LAP (3).
Note: Cell lines adhere to simple ligands (e.g. fibronectin) using more integrins than alphaV/beta6, so it would be necessary to also block other integrins (e.g. with anti-beta1 antibody) to see any effect of this antibody on adhesion to fibronectin. For example, H441 cells, which naturally express high levels of alphaV/beta6, may be used as a positive control.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No2Q871
FormSupplied as a liquid in PBS. No preservatives added.
PurityPurified by Protein A affinity chromatography.
ImmunogenImmunization of beta6 knockout mice with wild type mouse keratinocytes.
SpecificityRecognizes mouse integrin alphaV beta6. Blocks activity against mouse and human alphaV beta6. Does not recognize alphaV or other alphaV integrins. Species Crossreactivity: human and rat. Because the antibody was raised in knockout mice, it is likely to recognize the receptor in other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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