| Catalog # | I7661-38 |
| Applications | Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, FACS and ELISA. Other applications not tested. |
| Recommended Dilutions | Immunocytochemistry: 2ug/ml (use on numerous cell lines as long as Ca2+ is present), |
| Immunoprecipitation: 5ug/400ul lysate (0.5% triton PBS plus Ca++) |
| FACS: 2-5ug/10e6 cells, in the presence of Ca++. |
| Stimulation of cells: 2-10ug/ml per 10e6 cells. Binding buffer must contain 1mM Ca++ |
| Immunohistochemistry: For use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and paraffin embedded (1:500, citrate HEIR treated, Qin et al 2003). Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers. |
| Optimal dilution determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 2Q849 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.1. No preservatives. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Human IL-1b activated endothelial cells. |
| Specificity | Recognizes human Integrin beta 1. Reacts with calcium-dependent epitopes on the integrin b1 subunit. When bound to b1, activates b1 dependent binding to extra cellular matrix substrates. |
| Species Crossreactivity | Rabbit and monkey |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
