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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin beta1, Activating

Anti -Integrin beta1, Activating

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IP IH IC FC
Catalog #I7661-38
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, FACS and ELISA. Other applications not tested.
Recommended DilutionsImmunocytochemistry: 2ug/ml (use on numerous cell lines as long as Ca2+ is present),
Immunoprecipitation: 5ug/400ul lysate (0.5% triton PBS plus Ca++)
FACS: 2-5ug/10e6 cells, in the presence of Ca++.
Stimulation of cells: 2-10ug/ml per 10e6 cells. Binding buffer must contain 1mM Ca++
Immunohistochemistry: For use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and paraffin embedded (1:500, citrate HEIR treated, Qin et al 2003). Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q849
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.1. No preservatives.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman IL-1b activated endothelial cells.
SpecificityRecognizes human Integrin beta 1. Reacts with calcium-dependent epitopes on the integrin b1 subunit. When bound to b1, activates b1 dependent binding to extra cellular matrix substrates.
Species CrossreactivityRabbit and monkey
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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