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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)

Anti -Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC IF
CD29 is the integrin B1 chain which is expressed as heterodimer non-covalently associated with the integrin subunits 1 to 6 (CD49a to CD49f) and known as very late antigens (VLA). It is also called platelet GPlla. It has been reported as receptor of bronectin. The antigen mediates cellular adhesion primarily to collagen, laminin or fibronectin. It has a broad cellular reactivity but is not expressed by erythrocytes. In normal peripheral blood CD29 blood CD29 is found on approximately 41% of T cells, 40% of CD4+ T lymphocytes, 43% of CD8+ T lymphocytes (1). It is slightly reactive with peripheral B-cells. The co-expression of CD4 and CD29 allows the identi cation of the helper/inducer subpopulation of CD4 lymphocytes.
Catalog #I7661-39A
Applications Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (frozen only). Not assessed for Western Blotting.
Recommended DilutionImmunoprecipitation: Use Protein G; 2ug antibody/mg protein lysate.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.97
HostMouse
SourceHuman
Concentration~200ug/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.
Also available without BSA and azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman melanoma V+B2 cell line
SpecificityRecognizes a non-reduced protein of 110kDa and a reduced protein of 130kDa, identified as CD29 or the integrin b-1 in the family of cell adhesion receptors. Species Reactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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