Anti -Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IP FC N |
|
| Beta-1 integrin is a ubiquitously expressed ~89 kD type I transmembrane protein functioning as receptor when heterodimerized with one alpha subunit. It belongs to the integrin beta chain family consisting of four different genes, encoding multiple Beta-integrins via alternative splicing. Ligand-recognition depends on the composition of the heterodimer: either collagen, fibronectin, VCAM1, laminin, cytotactin, osteopontin, epiligrin, thrombospondin and CSPG4 can bind to the integrin-complex. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). | | | Catalog # | I7661-39D | | Applications | Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Functional Studies. Other applications have not been tested. | | Recommended Dilutions | Flow Cytometry: 1:50 Stains the extracellular domain of beta-1 integrin. Fix HUVEC cells in 4% paraformaldehyde before staining. | | Functional Studies: Functions as an inhibiting antibody. Functionally tested by adhesion assay (1), chemotaxis (2), patch clamp analysis (3) and neurotoxicity induced apoptosis (4). | | Optimal dilutions to be determined by the researcher. | | Positive Control | HT-29 colon carcinoma, Endothetlial cells | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 6A561 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, 0.1% BSA. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Human umbilical vein EC | | Specificity | Recognizes human beta1 integrin. Binds to several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells. Does not recognize a5b1 complex and not the cytoplasmic part of the beta1-subunit. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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