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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)

Anti -Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP FC N
Beta-1 integrin is a ubiquitously expressed ~89 kD type I transmembrane protein functioning as receptor when heterodimerized with one alpha subunit. It belongs to the integrin beta chain family consisting of four different genes, encoding multiple Beta-integrins via alternative splicing. Ligand-recognition depends on the composition of the heterodimer: either collagen, fibronectin, VCAM1, laminin, cytotactin, osteopontin, epiligrin, thrombospondin and CSPG4 can bind to the integrin-complex. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro).
Catalog #I7661-39D
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Functional Studies. Other applications have not been tested.
Recommended DilutionsFlow Cytometry: 1:50 Stains the extracellular domain of beta-1 integrin. Fix HUVEC cells in 4% paraformaldehyde before staining.
Functional Studies: Functions as an inhibiting antibody. Functionally tested by adhesion assay (1), chemotaxis (2), patch clamp analysis (3) and neurotoxicity induced apoptosis (4).
Optimal dilutions to be determined by the researcher.
Positive ControlHT-29 colon carcinoma, Endothetlial cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A561
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman umbilical vein EC
SpecificityRecognizes human beta1 integrin on tumor cells.. Binds to several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells. Does not recognize a5b1 complex and not the cytoplasmic part of the beta1-subunit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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