Suitable for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitatin, and Western Blot. Also suitable for inhibition of Jurkat binding to collagen and fibrinogen. Other applications have not been tested.

Flow Cytometry: 1:200-1:500 using live cells and goat anti-mouse secondary antibody. Immunocytochemistry: can be used with unfixed cells in culture. Oftentimes, fixing with 2-3% PFA for 5 minutes after primary antibody incubation will assist in obtaining strong signals since it fixes the primary antibodies to the tissues. Immunoprecipitation: 1:250-1:500. Western Blot: 1:1000-1:2000. (reducing, 115kD; non-reducing, 130kD) Inhibition of Jurkat binding to collagen and fibronectin: 1:100. Note: epitope is trypsin sensitive; cells should be prepared with PBS + EDTA (2mM) or other non-enzymatic methods. Blocking will not occur with trypsinized cells Note: Binding of antibody may be inhibited by magnesium ion concentrations greater than 0.5mM. Optimal dilution to be determined by the researcher.

Human lung, liver, and skeletal muscle tissues

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Purified beta 1 integrin and Jurkat cells

Supplied as a liquid.

Ascites

Recognizes human integrin beta 1. Epitope: aa82-87 of mature beta 1 integrin chain.