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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)

Anti -Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP IC FC
Catalog #I7661-39Q
ApplicationsSuitable for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitatin, and Western Blot. Also suitable for inhibition of Jurka binding to collagen and fibrinogen. Other applications have not been tested.
Recommended DilutionFACS: 1:200-1:500 using live cells and goat anti-mouse secondary antibody.
Immunocytochemistry: Antibody can be used with unfixed cells in culture. Oftentimes, fixing with 2-3% PFA for 5 minutes after primary antibody incubation will assist in obtaining strong signals since it fixes the primary antibodies to the tissues.
Immunoprecipitation: 1:250-1:500.
Western Blot: 1:1000-1:2000. (reducing, 115 kDa; non-reducing, 130 kDa)
Inhibition of Jurkat binding to collagen and fibronectin: 1:100. Note: epitope is trypsin sensitive; cells should be prepared with PBS + EDTA (2mM) or other non-enzymatic methods. Blocking will not occur with trypsinized cells
Note Binding of antibody may be inhibited by magnesium ion concentrations greater than 0.5mM.
Optimal dilution to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q845
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid (ascites).
PurityAscites
ImmunogenPurified beta 1 integrin and Jurkat cells
SpecificityRecognizes human integrin beta 1. Epitope: aa82-87 of mature beta 1 integrin chain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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