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Anti -Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)
Anti -Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
B IP IC FC |
|
| Catalog # | I7661-39Q | | Applications | Suitable for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitatin, and Western Blot. Also suitable for inhibition of Jurka binding to collagen and fibrinogen. Other applications have not been tested. | | Recommended Dilution | FACS: 1:200-1:500 using live cells and goat anti-mouse secondary antibody. | | Immunocytochemistry: Antibody can be used with unfixed cells in culture. Oftentimes, fixing with 2-3% PFA for 5 minutes after primary antibody incubation will assist in obtaining strong signals since it fixes the primary antibodies to the tissues. | | Immunoprecipitation: 1:250-1:500. | | Western Blot: 1:1000-1:2000. (reducing, 115 kDa; non-reducing, 130 kDa) | | Inhibition of Jurkat binding to collagen and fibronectin: 1:100. Note: epitope is trypsin sensitive; cells should be prepared with PBS + EDTA (2mM) or other non-enzymatic methods. Blocking will not occur with trypsinized cells | | Note | Binding of antibody may be inhibited by magnesium ion concentrations greater than 0.5mM. | | Optimal dilution to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 2Q845 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid (ascites). | | Purity | Ascites | | Immunogen | Purified beta 1 integrin and Jurkat cells | | Specificity | Recognizes human integrin beta 1. Epitope: aa82-87 of mature beta 1 integrin chain. | | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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