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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IC

Several major families of cell adhesion molecules can be distinguished. These include the cadherins and N-CAMs, which play a crucial role in cell recognition and adhesion, while integrins are implicated in the adherence of cells to the extracellular matrix. Cadherins constitute a family of proteins, the members of which are differentially expressed in the different tissues. One of the best characterized members is E-cadherin, which is prevalent in epithelial tissues. It has been shown to play a crucial role in the process of tumor cell metastasis. NCAM’s (neural cell adhesion molecules) exist in at least three forms of plasma membrane plycoproteins, which are expressed in a variety of tissues including most nerve cells.
The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an a- and a beta-subunit in interconnecting the cytoskeleton and the extracellular matrix.
Catalog #	I7661-40B
Applications	Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested.
Recommended Dilution	Immunohistochemistry (Frozen): 1:12.5-1:100 with avidin-biotinylated horseradish peroxidase complex as detection reagent.
	Western Blot: 1:50-1:500
	Optimum dilutions to be determined by the researcher.
Hybridoma	SP2/0 mouse myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	4A56
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, 0.09% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added.
Purity	Purified by Protein G affinity chromatography.
Immunogen	C-terminal 24 amino acids of Integrin beta 1D, including an appending N-terminal cysteine (CQENPIYKSPINNFKNPNYGRKAGL) coupled to KLH.
Specificity	Recognizes the cytoplasmic domain of the human Integrin subunit B1D present in cardiac and skeletal muscle. Species Crossreactivity: mouse and canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.