Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin beta 6

Anti -Integrin beta 6

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Integrin beta 6 (ITGB6) is a 95kD member of the integrin beta family. It forms noncovalent heterodimers with Integrin alpha V and appears on epithelia following injury or inflammation. It
activates TGF-beta and assists keratinocyte migration. Human Integrin beta 6 is a type I transmembrane glycoprotein that is 767aa in length. It contains a 688aa extracellular domain (ECD) (aa22-709) that incorporates a 241aa VWF-A domain. Over aa22-707, human Integrin beta 6 ECD shares 90% and 93% aa sequence identity with mouse and pig Integrin beta 6 ECD, respectively.
Catalog #I7661-44B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human Intergrin beta 6 is ~2.5ng/well.
Western Blot: 1ug/ml. Using a colormetric detection system, the detection limit for recombinant human Intergrin beta 6 is ~20ng/lane and 50ng/lane under non-reducing and reducing conditions, respectively.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No9H134
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa22-707 from human Integrin beta 6 expressed in CHO cells (P18564).
SpecificityRecognizes human Integrin beta 6. Shows 40% crossreactivity with recombinant human Integrin beta 2 under reducing conditions in Western Blot. Does not crossreact with recombinant human Integrin beta 1, 3, 5, 7 or 8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links