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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin beta 7

Anti -Integrin beta 7

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IC FC
Human Integrin beta 7 (also Integrin beta P and M290 IEL Ag) is a 120kD member of the integrin beta chain family of molecules. It is a type I transmembrane glycoprotein that contains a 704aa extracellular domain (ECD) (aa20-723) and a 52aa cytoplasmic tail. The ECD contains one von Willebrand factor A domain (aa150-389) and four cysteine-rich EGF-like repeats. One splice variant exists that shows a deletion of aa501-648. Integrin beta 7 forms a nondisulfide linked heterodimer with alpha 4 and alpha E integrins. Alpha 4 beta7 binds fibronectin, VCAM-1 and MADCAM1, while alpha E beta7 binds E-cadherin. The ECD of human beta 7 is 87% aa identical to the ECD of mouse beta 7.
Catalog #I7661-45C
ApplicationsSuitable for use in Flow Cytometry, Direct ELISA and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 0.2mg/ml using human peripheral blood lymphocytes. 10ul of the diluted solution labels 1-2.5x10e5 cells in a total reaction volume 200ul.
Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human Integrin beta 7 is ~1ng/well.
Immunocytochemistry: 10ug/ml to detect human Integrin beta 7 in huamn peripheral blood lymphocytes. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted I7661-45C overnight at 4° C followed by fluorochrome-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9H135
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa20-723f rom human Integrin beta 7 expressed in CHO cells. (P26010).
SpecificityRecognizes human Integrin beta 7.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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