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Specifications

Clone Host Grade Applications Monoclonal Rat Purified FC

Interferon- is an important immunoregulatory cytokine that plays a key role in host defense by exerting antiviral, antiproliferative and immunoregulatory activities. Bovine IFN- is synthesized as 166 amino acid (aa) precursor that contains a 20 aa signal sequence and a 146 aa mature region. Based on human studies, bovine IFN- exists as an -helical, noncovalently linked intercalated homodimer. In the mature segment, bovine IFN- is 61%, 79%, 97%, 45%, 44%, and 55% aa identical to human, porcine, ovine, rat, mouse, and cotton rat IFN- , respectively. The production of IFN- is upregulated synergistically by IL-12, IL- 18, IL-23, and IL-27. IFN- induces cytokine release and promotes the expression of various membrane proteins. This includes class I and II MHC antigens, Fc receptors, and leukocyte adhesion molecules. IFN- is a potent activator of macrophage effector functions. It also inhibits Th2 differentiation, stimulates Th1 development, and influences mononuclear cell chemotaxis. It influences chemotaxis by inducing the synthesis of CXC chemokines 9 and 10, and CC chemokines 2, 3, 4, and 5. The functional IFN- receptor complex consists of two distinct subunits. An subunit (IFN- R1) binds IFN- with high affinity, while a -subunit (IFN- R2) interacts with the IFN- occupied -subunit to transduce a signal. Each IFN- dimer interacts with two subunit receptor pairs.
Catalog #	I7661-66G
Applications	Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	7H28
Host	Rat
Source	Bovine
Concentration	As reported
Form	Supplied as a liquid. Conjugated to PE.
Purity	Purified immunoglobulin
Immunogen	Bovine IFNg
Specificity	Recognizes bovine IFNg.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.