Interferon alpha 2 (alpha 2a), Recombinant, Human (Interferon alpha-2, IFN-alpha-2, IFNa2, IFNa2a, Interferon alpha-A, IFN-alphaA, IFNA, LeIF A, MGC125764, MGC125765)
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| Recombinant protein corresponding to 165aa of human Interferon-alpha 2a expressed in E.coli is a single, non-glycosylated, polypeptide chain. | | | Catalog # | I7661-74Z | | Biological Activity | The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.7x10e8IU/mg. | | Dimers and Aggregates | <1% (SDS-PAGE) | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1.UV spectroscopy at 280nm using the absorbency value of 0.924 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). | | 2. Analysis by RP-HPLC, using a standard solution of IFN-alpha(2a) as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~19.2kD | | Source | E.coli | | Purity | ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug) | | Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Interferon alpha 2 (alpha 2a), Recombinant, Human (Interferon alpha-2, IFN-alpha-2, IFNa2, IFNa2a, Interferon alpha-A, IFN-alphaA, IFNA, LeIF A, MGC125764, MGC125765) |
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