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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interferon » Interferon alpha 2 (alpha 2b), Recombinant, Human (Interferon alpha-2, IFN-alpha-2, IFNa2, IFNa2b, Interferon alpha-A, IFN-alphaA, IFNA, LeIF A, MGC125764, MGC125765)

Interferon alpha 2 (alpha 2b), Recombinant, Human (Interferon alpha-2, IFN-alpha-2, IFNa2,
IFNa2b, Interferon alpha-A, IFN-alphaA, IFNA, LeIF A, MGC125764, MGC125765)

Pricing

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Specifications

Recombinant protein corresponding to 165aa of human Interferon-alpha 2b expressed in E.coli is a single, non-glycosylated, polypeptide chain.
Catalog #I7661-75C
Biological ActivityThe specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.6x10e8 IU/mg.
Protein ContentProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Recombinant corresponding to Interferon-alpha 2b as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~19.3kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration~1mg/ml
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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