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You are here:Home » Molecular Biology » Growth Factors-Interferon » Interferon alpha, Recombinant, Mouse, (CHO) (IFNa14-16)

Interferon alpha, Recombinant, Mouse, (CHO) (IFNa14-16)

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Specifications

This highly active interferon is constitutively produced by Chinese Hamster Ovary (CHO) cells which have been transfected with the hybrid interferon gene alpha 14-16 (alpha 1 AA 1-22 and alpha 4 AA 23-166). The hybrid alpha 1, alpha 4 gene product differs 6 amino acids from alpha 4 (AA 5, 7, 10, 17, 19 and 20). The AA 10, 17, 19 and 20 influence the antiviral response. Biological activity has been demonstrated in mouse as well as in hamster and rat systems. The natural alpha 4 interferon exhibits antiviral activity in mouse cells which is approximately six times higher than that exhibited by natural alpha 1 interferon. The activity is increased to a level which is ~100 times that of natural alpha 1 interferon and 15 times that of natural alpha 4 interferon.
The situation is somewhat different in hamster cells. The natural alpha 1 interferon is about 100 times more active than the natural alpha 4 interferon. The recombinant product is as active as the alpha 1 interferon.
These features are most probably due to the fact that at least two different receptors exist for alpha interferons in these systems. One receptor appears to be specific for the amino acids 10-20 whereas the other is specific for the amino acids 55-67. The recombinant product exposes or accesses both sites better, or at least as well as the parent proteins. In addition to the interferon alpha proteins' ability to protect cells against viral infection, they can also inhibit cell growth and possess immunomodulatory activities. These effects are mediated through binding to a specific cell surface receptor. Most of the interferon alpha species are not glycosylated and are further characterized by their acid stability at pH 2.
Catalog #I7661-88A
SourceRecombinant corresponding to a hybrid interferon gene alpha 14-16 (alpha 1 aa1-22 and alpha 4 aa23-166) expressed in CHO cell. The hybrid alpha 1, alpha 4 gene product differs 6aa from alpha 4 (aa5, 7, 10, 17, 19 and 20).
Unit DefinitionOne unit of mouse alpha interferon is the amount of alpha interferon which protects 50% of the indicator cell population from viral destruction. One unit of Mouse Alpha Interferon approximates the bioactivity contained in one unit of the mouse alpha interferon standard prepared by NIH (Ga02-901-511).
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceMouse, CHO-derived
PurityPurified
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS, 10% mouse albumin, 0.01% gelatin. Reconstitute by injection of 1ml ddH2O (Caution: vial is under vacuum).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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