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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified E FC N

Monoclonal antibodies directed against alpha Interferon make it possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These interferon activities can only be distinguished from one another by use of specific neutralizing antibodies.
Catalog #	I7662-02A
Applications	Suitable for use in ELISA, Flow Cytometry and Neutralization. Other applications have not been tested.
Recommended Dilution	Flow Cytometry: 1:50
	Neutralization of Biological Activity: 1ug per mouse i.v. before treatment with 35ug LPS i.p., decreased the LPS-induced IL-1beta response.
	Optimal dilutions to be determined by the researcher.
Recommended Positive Control	Plasmacytoid dendritic cells
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6A565
Host	Rat
Source	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, 0.1% BSA.
Purity	Purified by Protein G affinity chromatography.
Specificity	Recognizes both natural and recombinant mouse alpha Interferon. Reacts with most isoforms of natural or recombinant mouse alpha Interferons. Does not crossreact with mouse Interferons beta or gamma or with human Interferons.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.