Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Antibodies-Interferon » Anti -Interferon alpha (IFNa)

Anti -Interferon alpha (IFNa)


  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.


Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B FC N
Monoclonal antibodies directed against alpha Interferon make it possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These interferon activities can only be distinguished from one another by use of specific neutralizing antibodies.
Catalog #I7662-02A
ApplicationsSuitable for use in ELISA, Flow Cytometry, Western Blot and Neutralization. Other applications have not been tested.
Recommended DilutionsFlow Cytometry: 1:50. For intracellular staining of IFN-alpha, cells can be fixed in 1% formaldehyde; blocked and permeabilized in 0.2% saponin, 5% normal rabbit serum for 30 minutes on ice.
Neutralization of Biological Activity: 1ug per mouse i.v. before treatment with 35ug LPS i.p., decreased the LPS-induced IL-1beta response.
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlPlasmacytoid dendritic cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
Clone No6A565
FormSupplied as a liquid in PBS, 0.1% BSA.
PurityPurified by Protein G affinity chromatography.
SpecificityRecognizes both natural and recombinant mouse alpha Interferon. Reacts with most isoforms of natural or recombinant mouse alpha Interferons. Does not crossreact with mouse Interferons beta or gamma or with human Interferons.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

External Links