Suitable for use in Neutralization, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Optimal dilutions to be determined by the researcher.

One neutralization unit is the amount of antiserum required to neutralize one unit of mouse interferon beta to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) J. Virol. 37, 755-758] using L929 cells and EMCV as the challenge virus. In this antiviral assay for interferon, about 2.5unit/ml of interferon is the quantity necessary to produce acytopathic effect of 50%. The units are determined by use of a laboratory standard calibrated with respect to the international reference standard for Mu-IFN-beta provided by the National Institutes of Health [Gb02-902-511]. This materialis prepared specifically for effective neutralization of Mu-IFN-beta.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Recombinant corresponding to mouse interferon beta expressed in E.coli.

Supplied as a liquid in PBS.

Purified by immunoaffinity chromatography.

Recognizes mouse interferon beta.