| One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity. The synthesis of IFN-beta can be induced by common inducers of interferons, including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL-1 . In contrast to IFN-alpha, IFN-beta is strictly species-specific. IFN-beta of other species is inactive in human cells. IFN-beta is involved in the regulation of unspecific humoral immune responses and immune responses against viral infections. IFN-beta increases the expression of HLA class I antigens and blocks the expression of HLA class II antigens stimulated by IFN-gamma. |
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| Catalog # | I7662-14R |
| Recombinant Human Interferon-Beta 1a is a single, glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of 22500D. |
| Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu. |
| Biological Activity | Interferon Beta-1a is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 270 x 10e6 IU/ mg. |
| Dimers and Aggregates | < 1% (by silver-stained SDS-PAGE). |
| Protein Content | Protein quantitation was carried out by two independent methods: |
| 1. UV spectroscopy at 280nm. |
| 2. Analysis by RP-HPLC, using a standard solution of IFN-beta as a Reference Standard. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer |
| Molecular Weight | 22.5kD |
| Source | Recombinant, Human (CHO cells) |
| Purity | Chromatographically purified (> 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain). Endotoxin: 0.1ng/ug (IEU/ug). |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a lyophilized powder from 91mg Human albumin, 620mg mannitol, 9mg sodium acetate. |
| Reconstitute with sterile ddH2O to a concentration of .0.1mg/ml. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
