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You are here:Home » Molecular Biology » Growth Factors-Interferon » Interferon beta 1 (beta 1a), Recombinant, Human (IFNb1, IFNb1a)

Interferon beta 1 (beta 1a), Recombinant, Human (IFNb1, IFNb1a)

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Specifications

One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity. The synthesis of IFN-beta can be induced by common inducers of interferons, including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL-1 . In contrast to IFN-alpha, IFN-beta is strictly species-specific. IFN-beta of other species is inactive in human cells. IFN-beta is involved in the regulation of unspecific humoral immune responses and immune responses against viral infections. IFN-beta increases the expression of HLA class I antigens and blocks the expression of HLA class II antigens stimulated by IFN-gamma.
Catalog #I7662-14R
Recombinant Human Interferon-Beta 1a is a single, glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of 22500D.
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.
Biological ActivityInterferon Beta-1a is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 270 x 10e6 IU/ mg.
Dimers and Aggregates < 1% (by silver-stained SDS-PAGE).
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a standard solution of IFN-beta as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Molecular Weight22.5kD
SourceRecombinant, Human (CHO cells)
PurityChromatographically purified (> 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain). Endotoxin: 0.1ng/ug (IEU/ug).
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from 91mg Human albumin, 620mg mannitol, 9mg sodium acetate.
Reconstitute with sterile ddH2O to a concentration of .0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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