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Interferon beta 1 (beta 1b), Recombinant, Human (IFNb1, IFNb1b)
Interferon beta 1 (beta 1b), Recombinant, Human (IFNb1, IFNb1b)
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| One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity. The synthesis of IFN-beta can be induced by common inducers of interferons, including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL-1. | | | Catalog # | I7662-14S | | Recombinant Human IFN-beta 1b is a single, non-glycosylated mutein (variant form) of human Interferon beta-1b polypeptide chain containing 165 amino acids and having a molecular mass of 18.5kD. The Interferon beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17. | | Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu. | | Biological Activity | Interferon beta-1b is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 32x10e6IU/mg. | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). | | 2. Analysis by RP-HPLC, using a calibrated solution of IFN-beta as a Reference Standard. | | Reconstitution | Reconstitute the lyophilized Interferon beta-1b in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 18.5kD | | Source | E. coli | | Purity | 98% by RP-HPLC or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. | | Form | Supplied as a lyophilized powder containing 0mg/ml HSA, 50mg/ml dextrose. REconstitute with sterile ddH2O to a concentration >0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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