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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interferon » Interferon beta 1 (beta 1b), Recombinant, Human (IFNb1, IFNb1b)

Interferon beta 1 (beta 1b), Recombinant, Human (IFNb1, IFNb1b)

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Specifications

Recombinant protein corresponding to 165aa of human Interferon-beta 1b expressed in E.coli is a single, non-glycosylated mutein (variant form) of human Interferon beta-1b polypeptide chain.
Catalog #I7662-14T
Biological ActivityThe specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 32x10^6 IU/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IFN-beta as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~18.5kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug
Concentration~1mg/ml
FormSupplied as a lyophilized powder from a solution containing 50mg human albumin and 50mg dextrose. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration of 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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