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You are here:Home » Antibodies » Abs to Interferon » Anti -Interferon gamma (IFNg)

Anti -Interferon gamma (IFNg)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
IFNg induces a variety of biological responses, such as antiviral, antiproliferative and immunomodulatory activity in sensitive cells. Activation of the IFNg receptor (IFNgR) leads to auto- phosphorylation of the Janus kinases JAK1 and JAK2, and the nuclear translocation of the transcription factors Stat1a p91 and Stat1b p84. The IFNgR is composed of at least two chains,
designated IFNgR1 and IFNgR2. Although expression of IFNgR1 is sufficient for ligand binding, it alone does not confer responsiveness to IFNg. Concomitant expression of IFNgR1 and IFNgR2 is required for transcriptional activation of IFNg- inducible genes.
Catalog #I7662-15K
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionsELISA (Capture): 1-5ug/ml Dilute with appropriate coating buffer and coat each well with 100ul.
Optimal dilutions to be determined by the researcher.
HybridomaBALB/c mouse splenocytes were fused with NSO mouse myeloma cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5J18
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified recombinant human interferon-gamma (IFN-gamma).
SpecificityRecognizes natural and recombinant human interferon-gamma.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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