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You are here:Home » Antibodies » Abs to Interferon » Anti -Interferon gamma (IFNg)

Anti -Interferon gamma (IFNg)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified E B FC IF
Catalog #I7662-15M4
ApplicationsELISA Detection: Useful as a capture antibody in a sandwich ELISA for quantifying mouse IFNg protein levels. Biotinylated antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human IFNg as the standard.
Western Blot: Useful for Western blot applications. For Western blotting, a concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100ng/lane of recombinant mouse IFNg under reducing conditions.
Immunofluorescence/Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IFNg-positive cells within mixed cell populations.4-6 Recombinant mouse IFNg or Rat IgG1 should be used as a specificity control.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5K298
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
PurityPurified
ImmunogenE. coli-expressed recombinant mouse IFNg
SpecificityRecognizes mouse Interferon gamma (IFNg).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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