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You are here:Home » Antibodies » Abs to Interferon » Anti -Interferon gamma (IFNg) (Azide Free)

Anti -Interferon gamma (IFNg) (Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified E B FC IF N
Murine IFN-gamma is a regulatory protein produced by activated NK cells and CD4+TCR alpha-beta+, CD8+TCR alpha-beta+, and TCR gamma-delta+ T cells. Biological activities of IFN-gamma include specific binding to a single class of high affinity receptors.
Catalog #I7662-15M4X
Interferon gamma is the only known type II interferon. Under physiological conditions, interferon gamma is induced when T-lymphocytes, previously sensitized to an antigen, are exposed to the same antigen, hence its other name immune interferon. Interferon gamma has demonstrated significant anti-proliferative effects in in vivo studies; however, its therapeutic effects have been limited. Endogenous IFN-g has been shown to be required for the protection of mice against mycobacteri and leishmaniasis.
ApplicationsSuitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Neutralization. Other applications have not been tested.
Recommended DilutionELISA: Useful as a capture antibody in a sandwich ELISA. Use I7662-15M5 Interferon gamma (IFNg) (Biotin) as the detection antibody, with purified recombinant
mouse IL-17 as the standard.
Western Blot: 1-5ug/ml Using I1904-47P, IgG, H&L, X-Adsorbed (AP) Pab Gt x Rt detects of 100ng/lane of recombinant mouse IFNg under reducing conditions).
iImmunofluorescence: Use I1904-76U IgG1, Rat Isotype Control
Neutralization: Use I1904-76UX IgG1, Rat Isotype Control (Azide Free)
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5K298
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS. No preservative added.
PurityPurified
ImmunogenRecombinant mouse IFNg expressed E. coli.
SpecificityRecognizes mouse Interferon gamma (IFNg).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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