| Murine IFN-gamma is a regulatory protein produced by activated NK cells and CD4+TCR alpha-beta+, CD8+TCR alpha-beta+, and TCR gamma-delta+ T cells. Biological activities of IFN-gamma include specific binding to a single class of high affinity receptors. |
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| Catalog # | I7662-15M4X |
| Interferon gamma is the only known type II interferon. Under physiological conditions, interferon gamma is induced when T-lymphocytes, previously sensitized to an antigen, are exposed to the same antigen, hence its other name | immune interferon. Interferon gamma has demonstrated significant anti-proliferative effects in in vivo studies; however, its therapeutic effects have been limited. Endogenous IFN-g has been shown to be required for the protection of mice against mycobacteri and leishmaniasis. |
| Applications | Suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Neutralization. Other applications have not been tested. |
| Recommended Dilution | ELISA: Useful as a capture antibody in a sandwich ELISA. Use I7662-15M5 Interferon gamma (IFNg) (Biotin) as the detection antibody, with purified recombinant |
| mouse IL-17 as the standard. |
| Western Blot: 1-5ug/ml Using I1904-47P, IgG, H&L, X-Adsorbed (AP) Pab Gt x Rt detects of 100ng/lane of recombinant mouse IFNg under reducing conditions). |
| iImmunofluorescence: Use I1904-76U IgG1, Rat Isotype Control |
| Neutralization: Use I1904-76UX IgG1, Rat Isotype Control (Azide Free) |
| Optimal dilutions to be determined by researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 5K298 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS. No preservative added. |
| Purity | Purified |
| Immunogen | Recombinant mouse IFNg expressed E. coli. |
| Specificity | Recognizes mouse Interferon gamma (IFNg). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
