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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E IP FC IF

Catalog #	I7662-15Q1
Applications	ELISA Detection: Monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IFN-gamma protein levels.1,2 Biotinylated antibody should be paired with purified antibody as the capture antibody, with purified recombinant human IFN-g as the standard.
	Immunofluorescence/Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IFN-g-positive cells within mixed cell populations. Recombinant human IFN-g, unlabeled antibody, or Rat IgG1 should be used as a specificity control.
	Immunoprecipitation: Has been reported to be useful for immunoprecipitation.1
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	5K299
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Purity	Purified
Immunogen	E. coli-expressed recombinant human IFNg
Specificity	Recognizes human Interferon gamma (IFNg). Has been used for epitope mapping of human IFNg. Does not bind to denatured IFNg.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.