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You are here:Home » Antibodies » Abs to Interferon » Anti -Interferon gamma (IFNg)

Anti -Interferon gamma (IFNg)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E IP FC IF
Catalog #I7662-15Q1
ApplicationsELISA Detection: Monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IFN-gamma protein levels.1,2 Biotinylated antibody should be paired with purified antibody as the capture antibody, with purified recombinant human IFN-g as the standard.
Immunofluorescence/Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IFN-g-positive cells within mixed cell populations. Recombinant human IFN-g, unlabeled antibody, or Rat IgG1 should be used as a specificity control.
Immunoprecipitation: Has been reported to be useful for immunoprecipitation.1
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5K299
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
PurityPurified
ImmunogenE. coli-expressed recombinant human IFNg
SpecificityRecognizes human Interferon gamma (IFNg). Has been used for epitope mapping of human IFNg. Does not bind to denatured IFNg.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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