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You are here:Home » Antibodies » Abs to Interferon » Anti -Interferon gamma (IFNg) (Biotin)

Anti -Interferon gamma (IFNg) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
The cytokine human interferon-gamma (IFN-gamma) is a glycosylated protein that exists as a homodimer of 34kD in its biologically active form. IFN-gamma is expressed upon activation by TH1 cells CD8+ cells and NK cells (2). Secreted IFN-gamma is a potent inhibitor of in vitro hematopoiesis and appears to serve as an important regulator of lymphocyte and macrophage function by exerting antiproliferative effects (1).
Catalog #I7662-15Y2
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Protocol:
1. Ficoll prepared human peripheral blood lymphocytes were stimulated 4 hours in the presence of 50ng/ml PMA, 2uM ionomicin and 5uM monensin.
2. Cells were then harvested, washed and fixed for 30 min with 2% buffered formaldehyde after which they were washed two times.
3. Fixed cells were permeabolized 10 minutes in a buffer containing 0.3% Saponin. Subsequent reagent incubations and washes were done using this buffer.
4. Fivex10e5 cells per tube were preincubated 5 minutes with 20ul of 250ug/ml human IgG (To block nonspecific binding), after which they were incubated 45 minutes on ice with 8 ul of I7662-15Y2 at 1ug/ml.
5. Cells were washed twice and incubated with S7973-88P Streptavidin (PE) after which they were washed three times, fixed and analyzed using a BD FACS Calibur. A net 40% sub population of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml anti-IFN-gamma antibody.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.  
Clone TypeMonoclonal
IsotypeIgG1
Clone No7H148
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant human IFN-gamma
SpecificityBinds to recombinant and cellular IFN-gamma in ELISA and Flow cytometry.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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