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You are here:Home » Antibodies » Abs to Interferon » Anti -Interferon gamma (IFNg)

Anti -Interferon gamma (IFNg)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B N
Catalog #I7662-16B
ApplicationsSuitable for use in ELISA, Western Blot and Neutralization. Other applications not tested.
Recommended DilutionELISA (Indirect/Sandwich): 0.5-2ug/ml Detects recombinant human IFNg at 0.2-0.4ng/well using 100ul/well.
Western Blot: 0.1-0.2ug/ml. Detects recombinant human IFNg at 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Neutralization: 0.04-0.06ng/ml will yield one-half maximal inhibition of the biological activity of hIFNg (50ng/ml).
Optimal dilutions to be determined by the researcher..
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C Reconstitute with 40-50% glycerol, ddH2O. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 40-50% glycerol, ddH2O to 0.1-1mg/ml.
PurityPuri ed by af nity chromatography employing immobilized human IFNg matrix.
ImmunogenHighly pure (> 98%) recombinant human Interferon gamma
(IFNg).
SpecificityRecognizes human Interferon gamma (IFNg).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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