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You are here:Home » Antibodies » Abs to Interferon » Anti -Interferon gamma (IFNg) (Biotin)

Anti -Interferon gamma (IFNg) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Interferon gamma is the only known type II interferon. Under physiological conditions, interferon gamma is induced when T-lymphocytes, previously sensitized to an antigen, are exposed to the same antigen, hence its other name: immune interferon. Interferon gamma has demonstrated significant anti-proliferative effects in in vivo studies; however, its therapeutic effects have been limited. Endogenous IFN-gamma has been shown to be required for the protection of mice against mycobacteria and leishmaniasis. In the U.S., human IFN-gamma has been approved for reducing the frequency and severity of infections in patients with chronic granulomatous disease.
Catalog #I7662-16D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA (Direct): 0.15-0.30ug/ml, using 100ul/well antibody solution. Detects ~0.2ng/well. Sandwich: 0.25-1.0ug/ml , using 100ul/well antibody solution. Detects 0.2-0.4ng/well.
Western Blot: 0.1-0.2ug/ml, Detects 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS. Labeled with Biotin. Reconstitute with sterile PBS, 0.1% BSA to a concentration of 0.1-1mg/ml.
PurityPuri ed by immunoaf nity chromatography.
ImmunogenHighly pure (> 98%) recombinant human IFN-gamma
SpecificityRecognizes human IFN-gamma.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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