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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH N

IFN-gamma is a pluripotent cytokine with important pro-inflammatory functions.
Catalog #	I7662-16K
Applications	Can be used for Neutralization in bio-assays. Furthermore the antibody is useful for Immunohistochemical staining of IFN-gamma containing cells in frozen sections as well as in cells cultured on cover slips and for Western Blot and immunoassay.
Recommended Dilution	Immunohistology: 1:10
	Western Blot: 1:10
	For neutralization of biological activity dilutions have to be made according to the amounts IFN-gamma to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.
Purity	Purified IgG
Specificity	Reacts with rat Interferon gamma (IFN-gamma) of both natural and recombinant origin. Inhibits the biological activity of natural and recombinant rat IFN-gamma. Specificity was further assessed by ELISA and Western Blot. No crossreactivities with other cytokines have been detected.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.